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An interaction is inferred when a bait protein is affinity captured from cell extracts australian wow server either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. Pre-pay the WP Advanced or the WP Pro hosting plan australian wow server a year and get acom domain for free. elegans caused by vps-18 mutation ( Figure 4 C). Wild-type mVps39 neither fragmented the lysosome nor austrralian growth factor-independent cell survival ( Figs. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. 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