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Peptide-specific affinity-purified polyclonal anti-Rabenosyn-5 antibody has been previously described 16rabbit anti-hVps45, mouse anti-Syntaxin6, and mouse anti-Syntaxin16 were purchased from Synaptic Systems (Gottingen, Germany), mouse anti-actin and goat anti-hVps35 antibodies were from Abcam (Cambridge, MA), mouse anti-Rab5 and anti-SNX1 antibodies were from Transduction Laboratories (Lexington, KY), mouse anti-human О1 integrin was from Serotec (Oxford, UK), mouse anti-О-tubulin, mouse anti-GFP was from Roche Applied Science (Indianapolis, IN), mouse anti-HA was from Covance (Berkeley, CA), mouse anti-Myc was from Zymed Laboratories (Carlsbad, CA), and DAPI was from Molecular Probes (Carlsbad, CA). Yu Z, Gonciarz MD, Sundquist WI, Hill CP, Jensen GJ. A few companies toss in a SSL certificate free of comic hosting web others may charge you 100 for that extra layer of security. A platform built for developers. 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